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The use of Lenticules (TM) for the process control of enumeration techniques in food and environmental microbiology

Lookup NU author(s): Dr Nigel Lightfoot

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Abstract

Aims: Lenticules(TM) consist of control-dried plano convex discs in which biologically-active materials are contained within a water-soluble matrix. They can be produced to contain stable numbers of bacteria from 10 cfu lenticule(-1) to 10(8) cfu lenticule(-1) with a wide variety of, organisms. These experiments were carried, out to validate their use as a tool for internal quality control in quantitative microbiology. Methods and Results: The Lenticules(TM) were used routinely in standard quantitative microbiological procedures across five laboratories. Results showed the materials to be stable, homogenous and capable of identifying systematic errors. Conclusions: The Lenticules(TM) provide suitable, stable control materials. Significance and Impact of the Study: Routine internal quality control of quantitative measurements is greatly improved; the materials are easy to use and enable comparisons between laboratories to be made.

Publication metadata

Author(s): Lightfoot NF, Richardson IR, Harford JP

Publication type: Article

Publication status: Published

Journal: Journal of Applied Microbiology

Year: 2001

Volume: 91

Issue: 4

Pages: 660-667

ISSN (print): 1364-5072

ISSN (electronic): 1365-2672

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1365-2672.2001.01432.x

DOI: 10.1046/j.1365-2672.2001.01432.x

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