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A novel selective medium for the detection of methicillin-resistant Staphylococcus aureus enabling result reporting in under 24 h

Lookup NU author(s): Dr John Perry, Dr Katherine Orr

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Abstract

A simple selective broth was devised to indicate the presence of methicillin resistant Staphylococcus aureus (MRSA) in clinical samples. The broth comprised nutrient broth supplemented with sodium chloride, ciprofloxacin, colistin and aztreonam as selective agents and also mannitol, trehalose and phenol red as an indicator system. In a preliminary study using 228 clinical samples this selective mannitol broth (SMB) proved to be more sensitive than other selective agars for detection of MRSA within 24 h. In an extended study of a further 1124 clinical samples from 470 randomly selected patients, SMB detected 85.1% of MRSA strains present with a specificity of 43.6%. We conclude that SMB offers a convenient, inexpensive and sensitive method for high-throughput screening for MRSA. (C) 2002 The Hospital Infection Society.

Publication metadata

Author(s): Gurran C, Holliday MG, Perry JD, Ford M, Morgan S, Orr KE

Publication type: Article

Publication status: Published

Journal: Journal of Hospital Infection

Year: 2002

Volume: 52

Issue: 2

Pages: 148-151

ISSN (print): 0195-6701

ISSN (electronic): 1532-2939

Publisher: W.B. Saunders Co. Ltd.

URL: http://dx.doi.org/10.1053/jhin.2002.1260

DOI: 10.1053/jhin.2002.1260

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