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Candidate T cell epitopes of the human La/SSB autoantigen

Lookup NU author(s): Charlotte Gordon

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Abstract

Objective. To identify T cell epitopes of the human La autoantigen involved in the generation of anti-Ro/La autoantibodies. Methods. Molecular techniques were used for HLA typing of 219 white patients with systemic lupus erythematosus and 125 white patients with primary Sjogren's syndrome. Anti-Ro/La antibody levels were measured by enzyme-linked immunosorbent assay. Peripheral blood mononuclear cell responses to an overlapping series of synthetic 15-mer peptides spanning the entire La sequence were examined in pools or individually in conventional 7-day proliferation assays. Results. HLA typing confirmed that the HLA-DR3/DQ2 haplotype is closely associated with the occurrence of anti-Ro/La antibodies, and that the frequency of HLA-DR1 and DR4 haplotypes is reduced among antibody-positive patients. We identified 3 regions of the La sequence likely to contain T cell epitopes and I peptide, La 49-63, that generated a low-level but clear-cut T cell proliferative response. The HLA restrictions of these responses mirrored the HLA association data from the cohort study. Among individuals who were HLA-DR3 positive, there was no difference between patients and controls in the proliferative response to the La 49-63 peptide. Conclusion. Our data suggest that these are naive T cell responses, and that the identification of T cell epitopes involved in the generation of anti-Ro/La autoantibodies should focus on alternative candidate antigens.

Publication metadata

Author(s): Davies ML, Taylor EJ, Gordon C, Young SP, Welsh K, Bunce M, Wordsworth BP, Davidson B, Bowman SJ

Publication type: Article

Publication status: Published

Journal: Arthritis & Rheumatism

Year: 2002

Volume: 46

Issue: 1

Pages: 209-214

ISSN (print): 0004-3591

ISSN (electronic): 1529-0131

Publisher: John Wiley & Sons, Inc.

URL: http://dx.doi.org/10.1002/1529-0131(200201)46:1<209::AID-ART10066>3.0.CO;2-1

DOI: 10.1002/1529-0131(200201)46:1<209::AID-ART10066>3.0.CO;2-1

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