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Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

Lookup NU author(s): Robert Griffiths, Professor Anthony O'Donnell

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Abstract

A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

Publication metadata

Author(s): Griffiths RI; O'Donnell AG; Whiteley AS; Bailey MJ

Publication type: Article

Publication status: Published

Journal: Applied and Environmental Microbiology

Year: 2000

Volume: 66

Issue: 12

Pages: 5488-5491

ISSN (print): 0099-2240

ISSN (electronic): 1098-5336

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/AEM.66.12.5488-5491.2000

DOI: 10.1128/AEM.66.12.5488-5491.2000

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