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Identification of an HLA-B7 serological variant and its characterization by sequencing based typing

Lookup NU author(s): Gary Cavanagh

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Abstract

We have identified an HLA-B*07 variant allele, B*0716, in a Caucasoid cadaver kidney donor. The HLA class I type by polymerase chain reaction using sequence-specific primers (PCR-SSP) was A*01, 32; B*07, 08; Cw*07. Serological typing, using monoclonal and polyclonal anti-HLA antisera, gave disparate results for the B antigens. Monoclonal antibodies identified B7 and B8 antigens but polyclonal antisera recognised only the B8 antigen. PCR using sequencing based typing (PCR-SBT) confirmed the presence of both B*0703 and B*0801 alleles but with a mutation in one of the alleles. The HLA-B*07 allele was isolated by allele-specific PCR and was shown to have a mutation, G-->T, at 292 in exon 2. This mutation changes codon 74, which encodes aspartic acid (GAC) present in all previously identified B*07 alleles, to tyrosine (TAC) in the variant. The serological results suggest that codon 74 is a crucial part of a B7 antigen-specific epitope recognised by tissue typing polyclonal antisera.

Publication metadata

Author(s): Dunn PPJ, Carter V, Dunn A, Day S, Fuggle SV, Ross J, Cavanagh G

Publication type: Article

Publication status: Published

Journal: Tissue Antigens

Year: 2000

Volume: 55

Issue: 1

Pages: 71-73

ISSN (print): 0001-2815

ISSN (electronic): 1399-0039

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1034/j.1399-0039.2000.550114.x

DOI: 10.1034/j.1399-0039.2000.550114.x

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