Toggle Main Menu Toggle Search

Open Access padlockePrints

The Newcastle University research output collection, currently available on ePrints, will shortly be moving to a new open repository platform, Figshare. To prepare for the data migration we have paused adding new content to ePrints, and will resume once the new repository is launched. During this time you will continue to have access to ePrints (but no new content will appear). We will share updates here when available.

Expression and localisation of the pyrophosphate transporter, ANK, in murine kidney cells

Lookup NU author(s): Dr Georgina Carr, Professor John SayerORCiD, Professor Nicholas Simmons

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Background/Aims: Mutation of the pyrophosphate transporter, ANK, results in progressive arthritis in mice. ANK is expressed in non-skeletal tissues including kidney. The aim was therefore to investigate ANK location at the cellular and subcellular level in renal cells. Methods: RT-PCR identified a murine cell-line, mIMCD3, expressing ANK. The intra-renal distribution of ANK was determined by immunohistochemistry and the subcellular distribution in mIMCD3 cells by transfection of an ANK-NT-GFP fusion protein. Furthermore, an inactivating mutation of murine ank, Glu440X, and a gain of function mutation, Met48Thr, were tested to determine whether membrane traffic contributed to a transport defect. Results: ANK is expressed in cells of the cortical collecting duct, as assessed by colocalisation with aquaporin 2 and at the lateral and apical plasma membranes of mIMCD-3 epithelial cells, as assessed by colocalisation with wheat germ agglutinin lectin (WGA). ANK-NT-GFP was also present in endoplasmic reticulum, Golgi, acidic endosomes and mitochondria. mIMCD3 expression of Glu440X ANK-NT-GFP shows evidence of Golgi retention whereas Met48Thr ANK-NT-GFP is unaltered at the plasma membrane compared to wild type. Conclusion: The intra-renal and subcellular localisation of ANK is consistent with pyrophosphate export from collecting duct cells and supports a role for ANK in limiting intra-renal calcium-crystal formation. Copyright © 2007 S. Karger AG.

Publication metadata

Author(s): Carr G, Sayer JA, Simmons NL

Publication type: Article

Publication status: Published

Journal: Cellular Physiology and Biochemistry

Year: 2007

Volume: 20

Issue: 5

Pages: 507-516

Print publication date: 01/01/2007

ISSN (print): 1015-8987

ISSN (electronic): 1421-9778

Publisher: S. Karger AG

URL: http://dx.doi.org/10.1159/000107534

DOI: 10.1159/000107534

PubMed id: 17762177

Altmetrics

Altmetrics provided by Altmetric

Share