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Measurement of protein-like fluorescence in river and waste water using a handheld spectrophotometer

Lookup NU author(s): Eleanor Simpson, Malcolm Slater

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Abstract

Protein-like fluorescence intensity in rivers increases with increasing anthropogenic DOM inputs from sewerage and farm wastes. Here, a portable luminescence spectrophotometer was used to investigate if this technology could be used to provide both field scientists with a rapid pollution monitoring tool and process control engineers with a portable waste water monitoring device, through the measurement of river and waste water tryptophan-like fluorescence from a range of rivers in NE England and from effluents from within two waste water treatment plants. The portable spectrophotometer determined that waste waters and sewerage effluents had the highest tryptophan-like fluorescence intensity, urban streams had an intermediate tryptophan-like fluorescence intensity, and the upstream river samples of good water quality the lowest tryptophan-like fluorescence intensity. Replicate samples demonstrated that fluorescence intensity is reproducible to ±20% for low fluorescence, 'clean' river water samples and ±5% for urban water and waste waters. Correlations between fluorescence measured by the portable spectrophotometer with a conventional bench machine were 0.91; (Spearman's rho, n=143), demonstrating that the portable spectrophotometer does correlate with tryptophan-like fluorescence intensity measured using the bench spectrophotometer. © 2004 Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Baker A, Ward D, Lieten SH, Periera R, Simpson EC, Slater M

Publication type: Article

Publication status: Published

Journal: Water Research

Year: 2004

Volume: 38

Issue: 12

Pages: 2934-2938

ISSN (print): 0043-1354

ISSN (electronic): 1879-2448

Publisher: IWA Publishing

URL: http://dx.doi.org/10.1016/j.watres.2004.04.023

DOI: 10.1016/j.watres.2004.04.023

PubMed id: 15223288

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