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Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Lookup NU author(s): Dr Eva Spitalska, Dr Olivier Sparagano

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Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples. © Springer-Verlag 2004.

Publication metadata

Author(s): Spitalska E, Torina A, Cannella V, Caracappa S, Sparagano OAE

Publication type: Article

Publication status: Published

Journal: Parasitology Research

Year: 2004

Volume: 94

Issue: 4

Pages: 318-320

Print publication date: 01/10/2004

ISSN (print): 0932-0113

ISSN (electronic): 1432-1955

Publisher: Springer

URL: http://dx.doi.org/10.1007/s00436-004-1217-2

DOI: 10.1007/s00436-004-1217-2

PubMed id: 15372232

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