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Salmonella typhimurium as a basis for a live oral Echinococcus granulosus vaccine

Lookup NU author(s): Professor Carlos Hormaeche

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Abstract

A live attenuated Salmonella typhimurium vaccine candidate, LVR01, was constructed by introducing a null deletion into the aroC gene of the parental canine S. typhimurium isolate, P228067. LVR01 was used to orally deliver to the canine immune system a fatty acid binding protein (FABP) from Echinococcus granulosus (EgDf1), as a fusion protein with fragment C (TetC) of tetanus toxin. Immunization studies demonstrated that live LVR01 is well tolerated by orally vaccinated dogs. There was no detectable shedding of the vaccine strain in the faeces 2 days after immunization. Humoral antibody responses were observed against Salmonella, TetC and EgDf1. Cellular responses were consistently detected against Salmonella and TetC. A cellular response against EgDf1 was also seen in a proportion of the LVR01 vaccinated dogs. We propose S. typhimurium LVR01 as a carrier for recombinant antigens and a vector for the construction of multivalent oral vaccines for dogs. (C) 2000 Elsevier Science Ltd.

Publication metadata

Author(s): Chabalgoity JA, Moreno M, Carol H, Dougan G, Hormaeche CE

Publication type: Article

Publication status: Published

Journal: Vaccine

Year: 2000

Volume: 19

Issue: 4-5

Pages: 460-469

ISSN (print): 0264-410X

ISSN (electronic): 1873-2518

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/S0264-410X(00)00197-3

DOI: 10.1016/S0264-410X(00)00197-3

PubMed id: 11027809

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