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The Bacillus secretion stress response is an indicator for alpha-amylase production levels

Lookup NU author(s): Dr Jan-Willem Veening, Dr Sierd Bron

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Abstract

Aims: Overproduced α-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level α-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to α-amylase production.Methods and Results: The expression of a transcriptional htrB–lacZ gene fusion, and the levels of α-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of α-amylase production.Conclusion: Our observations show that the secretion stress response can serve as an indicator for α-amylase production levels.Significance and Impact of Study: Conceivably, this stress response can be employed to monitor the biotechnological production of various secretory proteins by the Bacillus cell factory.

Publication metadata

Author(s): Westers H, Darmon E, Zanen G, Veening JW, Kuipers OP, Bron S, Quax WJ, van Dijl JM

Publication type: Article

Publication status: Published

Journal: Letters in Applied Microbiology

Year: 2004

Volume: 39

Issue: 1

Pages: 65-73

ISSN (print): 0266-8254

ISSN (electronic): 1472-765X

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1111/j.1472-765X.2004.01539.x

DOI: 10.1111/j.1472-765X.2004.01539.x

PubMed id: 15189290

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