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Characterisation of Chlamydia psittaci isolated from a horse

Lookup NU author(s): Dr Meryl Lusher, Dr Sam Richmond

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Abstract

This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elemenrary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.

Publication metadata

Author(s): Wills JM, Watson G, Lusher ME, Mair TS, Wood D, Richmond SJ

Publication type: Article

Publication status: Published

Journal: Veterinary Microbiology

Year: 1990

Volume: 24

Issue: 1

Pages: 11-19

Print publication date: 01/07/1990

ISSN (print): 0378-1135

ISSN (electronic): 1873-2542

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/0378-1135(90)90046-X

DOI: 10.1016/0378-1135(90)90046-X

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