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Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain

Lookup NU author(s): Dr Mark EldridgeORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© 2026 The Author(s).Viral injections in nonhuman primates allow modification of neurons to investigate the organization and function of neural circuits, their developmental processes, and disease-related mechanisms. Here, we present a protocol for performing precise stereotaxic microinjections in the macaque brain. We describe steps for pre-surgical imaging, target planning, viral injections, and perioperative care. We then detail procedures for tissue extraction and slice electrophysiology experiments in multiple subcortical and cortical regions. For complete details on the use and execution of this protocol, please refer to Furlanis et al. (https://doi.org/10.1016/j.neuron.2025.05.002).

Publication metadata

Author(s): Tangen AC, Harmon P, Plotnikova AS, Mohanty A, Cummins AC, Pelkey KA, Cameron K, Rood C, Guerriero KA, McBain CJ, Eldridge MAG, Averbeck BB, Azadi R

Publication type: Article

Publication status: Published

Journal: STAR Protocols

Year: 2026

Volume: 7

Issue: 2

Print publication date: 19/06/2026

Online publication date: 28/04/2026

Acceptance date: 02/04/2018

Date deposited: 12/05/2026

ISSN (electronic): 2666-1667

Publisher: Cell Press

URL: https://doi.org/10.1016/j.xpro.2026.104522

DOI: 10.1016/j.xpro.2026.104522

Data Access Statement: All data reported in this paper will be shared by the lead contact upon request. All code used in this paper will be shared by the lead contact upon request. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

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