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Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading.

Lookup NU author(s): Dr Charles WinterhalterORCiD, Dr Kathy StrattonORCiD, Dr Stepan FenykORCiD, Professor Heath MurrayORCiD

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Author(s): Winterhalter C, Stratton KJ, Fenyk S, Murray H

Publication type: Article

Publication status: Published

Journal: Nature Communications

Year: 2025

Volume: 16

Print publication date: 30/12/2025

Online publication date: 26/11/2025

Acceptance date: 04/11/2025

Date deposited: 16/12/2025

ISSN (print): 0028-0836

ISSN (electronic): 2041-1723

Publisher: Nature Publishing Group

URL: https://doi.org/10.1038/s41467-025-66550-w

DOI: 10.1038/s41467-025-66550-w

Data Access Statement: The SSB:ssDNA and SSB:RecO models generated by AlphaFold 3 in this study have been deposited in Zenodo (https://zenodo.org/records/14793919) and in ModelArchive (https://modelarchive.org/) with the accession codes ma-tnuut (https://modelarchive.org/doi/10.5452/ma-tnuut) and ma-2k00a (https://modelarchive.org/doi/10.5452/ma-2k00a). Raw sequencing data has been deposited to the European Nucleotide Archive (ENA) (https://www.ebi.ac.uk/ena/browser/home) with the accession code PRJEB102268 (https://www.ebi.ac.uk/ena/browser/text-search?query=PRJEB102268). For additional information and requests, please contact the corresponding authors. Source data are available on Figshare (https://doi.org/10.6084/m9.figshare.30118162) and are provided with this paper.

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