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OT-Mation: an open-source code for parsing CSV files into Python scripts for control of OT-2 liquid-handling robotics

Lookup NU author(s): Alex Laverick, Katherine Anne Convey, Dr Catherine Harrison, Dr James StachORCiD, Dr Thomas Howard

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Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© 2025 The Author(s).OT-Mation is an open-source Python script designed to automate the programming of OT-2 liquid-handling robots, making combinatorial experiments more accessible to researchers. By parsing user-defined CSV files containing information on labware, reagents, pipettes, and experimental design, OT-Mation generates a bespoke Python script compatible with the OT-2 system. OT-Mation enhances reproducibility, reduces human error, and streamlines workflows, making it a valuable addition to any laboratory utilizing OT-2 robotics for liquid handling. While OT-Mation can be used for setting up any type of experiment on the OT-2, its real utility lies in making the connection between multifactorial experimental design software outputs (i.e. design of experiments arrays) and liquid-handling robot executable code. As such, OT-Mation helps bridge the gap between code-based flexibility and user-friendly operation, allowing researchers with limited programming skills to design and execute complex experiments efficiently. Graphical Abstract

Publication metadata

Author(s): Laverick A, Convey K, Harrison C, Tomlinson J, Stach J, Howard TP

Publication type: Article

Publication status: Published

Journal: Synthetic Biology

Year: 2025

Volume: 10

Issue: 1

Online publication date: 11/04/2025

Acceptance date: 08/04/2025

Date deposited: 20/05/2025

ISSN (print): 1939-7267

ISSN (electronic): 2397-7000

Publisher: Oxford University Press

URL: https://doi.org/10.1093/synbio/ysaf009

DOI: 10.1093/synbio/ysaf009

Data Access Statement: Code, documentation, example fles, and installation instructions can be found at https://github.com/tphoward/OT-Mation_Repo/

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Funding

Funder referenceFunder name
BB/W01095X/1
BBSRC

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