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Use of Population-Based Compartmental Modeling and Retinol Isotope Dilution to Study Vitamin A Kinetics and Total Body Stores among Ghanaian Women of Reproductive Age

Lookup NU author(s): Professor Georg LietzORCiD, Dr Anthony OxleyORCiD

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Abstract

© 2024 The Author(s)Background: Limited data are available on vitamin A kinetics and total body stores (TBS) in women. Such information can be obtained using compartmental modeling and retinol isotope dilution (RID). Objectives: Objectives were to apply population-based (“super-subject”) modeling to determine retinol kinetics in nonpregnant Ghanaian women of reproductive age and to use RID to predict TBS in the group and its individuals. Methods: Women (n = 89) ingested a dose of [2H6]retinyl acetate and blood samples (3/woman) were collected from 6 h to 91 d, with all participants sampled at 14 d, about half at either 21 or 28 d, and each at one other time. Composite data (plasma retinol fraction of dose; FDp) were analyzed using Simulation, Analysis and Modeling software to obtain kinetic parameters, TBS, and other state variables as well as model-derived values for the RID composite coefficient FaS. The latter were used in the RID equation TBS (μmol) = FaS × 1/SAp (where SAp is plasma retinol specific activity) to predict TBS at various times. Results: Model-predicted TBS was 973 μmol (n = 87). Geometric mean RID-predicted TBS was 965, 926, and 1006 μmol at 14, 21, and 28 d, respectively, with wide ranges [for example, 252–3848 μmol on day 14 (n = 86)]; TBS predictions were similar at later times. Participants had a mean 2 y of vitamin A in stores and estimated liver vitamin A concentrations in the normal range. Model-predicted vitamin A disposal rate was 1.3 μmol/d and plasma recycling number was 37. Conclusions: Super-subject modeling provides an estimate of group mean TBS as well as group-specific values for the RID coefficient FaS; the latter can be used to confidently predict TBS by RID for individual participants in the group under study or in similar individuals at 14 d or more after isotope ingestion. Trial registration number: Trial is registered (NCT04632771) at https://clinicaltrials.gov.

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Author(s): Green MH, Lopez-Teros V, Green JB, Lietz G, Kumordzie SM, Oxley A, Fuseini AD, Nyaaba KW, Becher E, Davis JN, Wessells KR, Adu-Afarwuah S, Engle-Stone R, Haskell MJ

Publication type: Article

Publication status: Published

Journal: Current Developments in Nutrition

Year: 2024

Volume: 8

Issue: 11

Print publication date: 01/11/2024

Online publication date: 17/10/2024

Acceptance date: 15/10/2024

Date deposited: 02/12/2024

ISSN (electronic): 2475-2991

Publisher: Elsevier B.V.

URL: https://doi.org/10.1016/j.cdnut.2024.104484

DOI: 10.1016/j.cdnut.2024.104484

Data Access Statement: Data are available upon request from the corresponding authors

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