Assessing MicroRNA Profiles from Low Concentration Extracellular Vesicle RNA Utilizing NanoString nCounter Technology

Crossland, RE; Macdonald, J; Wang, X-N

doi:10.1007/978-1-0716-3918-4_9

Assessing MicroRNA Profiles from Low Concentration Extracellular Vesicle RNA Utilizing NanoString nCounter Technology

Lookup NU author(s): Dr Rachel Crossland ORCiD, Jamie Macdonald, Dr Xiao Wang ORCiD

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Abstract

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Extracellular vesicles (EV) are rich in small RNA; however, a frequent caveat can be low abundance of EV RNA content, especially in clinical studies. NanoString MicroRNA Assays allow for multiplexed profiling of n = 800 mature microRNAs and can be applied to assess EV microRNA cargo. Here, we describe a method to adapt NanoString nCounter microRNA profiling to assess mature microRNA expression in low-concentration RNA samples, including concentrating the RNA, quantifying the RNA, and performing the NanoString protocol. Twelve samples can be assessed at one time using this method.