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Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients

Lookup NU author(s): Dr Corey ChanORCiD, Dr Kenneth RankinORCiD

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Abstract

© 2023, The Author(s), under exclusive licence to Springer Nature Limited. The surgical resection of solid tumours can be enhanced by fluorescence-guided imaging. However, variable tumour uptake and incomplete clearance of fluorescent dyes reduces the accuracy of distinguishing tumour from normal tissue via conventional fluorescence intensity-based imaging. Here we show that, after systemic injection of the near-infrared dye indocyanine green in patients with various types of solid tumour, the fluorescence lifetime (FLT) of tumour tissue is longer than the FLT of non-cancerous tissue. This tumour-specific shift in FLT can be used to distinguish tumours from normal tissue with an accuracy of over 97% across tumour types, and can be visualized at the cellular level using microscopy and in larger specimens through wide-field imaging. Unlike fluorescence intensity, which depends on imaging-system parameters, tissue depth and the amount of dye taken up by tumours, FLT is a photophysical property that is largely independent of these factors. FLT imaging with indocyanine green may improve the accuracy of cancer surgeries.

Publication metadata

Author(s): Pal R, Lwin TM, Krishnamoorthy M, Collins HR, Chan CD, Prilutskiy A, Nasrallah MLP, Dijkhuis TH, Shukla S, Kendall AL, Marshall MS, Carp SA, Hung YP, Shih AR, Martinez-Lage M, Zukerberg L, Sadow PM, Faquin WC, Nahed BV, Feng AL, Emerick KS, Mieog JSD, Vahrmeijer AL, Rajasekaran K, Lee JYK, Rankin KS, Lozano-Calderon S, Varvares MA, Tanabe KK, Kumar ATN

Publication type: Article

Publication status: Published

Journal: Nature Biomedical Engineering

Year: 2023

Volume: 7

Pages: 1649–1666

Online publication date: 16/10/2023

Acceptance date: 10/09/2023

ISSN (electronic): 2157-846X

Publisher: Springer Nature

URL: https://doi.org/10.1038/s41551-023-01105-2

DOI: 10.1038/s41551-023-01105-2

PubMed id: 37845517

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