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A novel glycosidase plate-based assay for the quantification of galactosylation and sialylation on human IgG

Lookup NU author(s): Dr David BolamORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© 2020, The Author(s). Changes in human IgG galactosylation and sialylation have been associated with several inflammatory diseases which are a major burden on the health care system. A large body of work on well-established glycomic and glycopeptidomic assays has repeatedly demonstrated inflammation-induced changes in IgG glycosylation. However, these assays are usually based on specialized analytical instrumentation which could be considered a technical barrier for uptake by some laboratories. Hence there is a growing demand for simple biochemical assays for analyzing these glycosylation changes. We have addressed this need by introducing a novel glycosidase plate-based assay for the absolute quantification of galactosylation and sialylation on IgG. IgG glycoproteins are treated with specific exoglycosidases to release the galactose and/or sialic acid residues. The released galactose monosaccharides are subsequently used in an enzymatic redox reaction that produces a fluorescence signal that is quantitative for the amount of galactosylation and, in-turn, sialylation on IgG. The glycosidase plate-based assay has the potential to be a simple, initial screening assay or an alternative assay to the usage of high-end analytical platforms such as HILIC-FLD-MSn when considering the analysis of galactosylation and sialylation on IgG. We have demonstrated this by comparing our assay to an industrial established HILIC-FLD-MSn glycomic analysis of 15 patient samples and obtained a Pearson’s r correlation coefficient of 0.8208 between the two methods.

Publication metadata

Author(s): Rebello OD, Gardner RA, Urbanowicz PA, Bolam DN, Crouch LI, Falck D, Spencer DIR

Publication type: Article

Publication status: Published

Journal: Glycoconjugate Journal

Year: 2020

Volume: 37

Pages: 691-702

Print publication date: 01/12/2020

Online publication date: 16/10/2020

Acceptance date: 06/10/2020

Date deposited: 12/07/2021

ISSN (print): 0282-0080

ISSN (electronic): 1573-4986

Publisher: Springer Nature

URL: https://doi.org/10.1007/s10719-020-09953-9

DOI: 10.1007/s10719-020-09953-9

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