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MicroRNA expression profile in the vitreous of proliferative diabetic retinopathy patients and differences from patienttreated with anti-VEGF therapy

Lookup NU author(s): David Steel

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Abstract

© 2020 The Authors.Purpose: microRNAs (miRNAs) mediate the pathological mechanisms of diabetic retinopathy. In this study, we compared miRNA expression profiles in the vitreous between patients with proliferative diabetic retinopathy (PDR) and patients with a macular hole as non-diabetic controls, and between PDR patients treated with antivascular endothelial growth factor (VEGF) therapy and untreated PDR patients. Methods: Vitreous samples of non-diabetic and PDR patients were screened for miRNAs with quantitative polymerase chain reaction (qPCR) panels. miRNA candidates were validated in vitreous samples of a second, independent cohort. In addition, the effect of anti-VEGF therapy was investigated in the vitreous of a third study population consisting of PDR patients who had not received anti-VEGF therapy and PDR patients who had received preoperative anti-VEGF therapy. Results: During screening, seven miRNAs were found to be significantly higher in the vitreous of PDR patients, whereas two miRNAs were found to be significantly lower compared with non-diabetic controls. Validating the expression of these miRNAs in a second cohort resulted in the identification of six miRNAs that were expressed at significantly higher rates in the vitreous of PDR patients: hsa-miR-20a-5p, hsa-miR-23b3p, hsa-miR-142-3p, hsa-miR-185-5p, hsa-miR-326, and hsa-miR-362-5p. Among these six miRNAs, hsa-miR-23b-3p levels were lower in the anti-VEGF-treated group of PDR patients compared with untreated PDR patients. Conclusions: In this study, we identified six miRNAs that are expressed more highly in PDR patients and one miRNA that is expressed at a lower levels in anti-VEGF-treated PDR patients. Translational Relevance: miRNAs identified in the vitreous of PDR patients may improve our understanding of the mechanisms leading to PDR.

Publication metadata

Author(s): Friedrich J, Steel DHW, Schlingemann RO, Koss MJ, Hammes H-P, Krenning G, Klaassen I

Publication type: Article

Publication status: Published

Journal: Translational Vision Science and Technology

Year: 2020

Volume: 9

Issue: 6

Pages: 1-10

Print publication date: 01/05/2020

Online publication date: 19/05/2020

Acceptance date: 23/03/2020

Date deposited: 17/08/2020

ISSN (electronic): 2164-2591

Publisher: Association for Research in Vision and Ophthalmology Inc.

URL: https://doi.org/10.1167/tvst.9.6.16

DOI: 10.1167/tvst.9.6.16

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