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Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties

Lookup NU author(s): Elena Mancuso, Dr Piergiorgio GentileORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic-resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects; (2) rediscovering the beneficial effects of naturally derived agents; and (3) preserving the substrate functional properties. For the first time, Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer assembly. Biomimetic electrospun poly(εcaprolactone) meshes were treated via layer-by-layer assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physicochemical characterization demonstrated the successful nanocoating formation. Different cell lines (human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated that the antimicrobial MH activity was dependent on the concentration used and strains tested.

Publication metadata

Author(s): Mancuso E, Tonda-Turo C, Ceresa C, Pensabene V, Connell SD, Fracchia L, Gentile P

Publication type: Article

Publication status: Published

Journal: Frontiers in Bioengineering and Biotechnology

Year: 2019

Volume: 7

Print publication date: 04/12/2019

Online publication date: 04/12/2019

Acceptance date: 06/11/2019

Date deposited: 13/12/2019

ISSN (electronic): 2296-4185

Publisher: Frontiers Research Foundation

URL: https://doi.org/10.3389/fbioe.2019.00344

DOI: 10.3389/fbioe.2019.00344

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