An<em> In Vitro</em> Human Skin Test for Assessing Adverse Immune Reactions and Sensitization Potential

Dickinson, A; Nong, Wang, X; Ahmed, S

doi:10.1007/978-3-319-50353-0_30

An In Vitro Human Skin Test for Assessing Adverse Immune Reactions and Sensitization Potential

Lookup NU author(s): Professor Anne Dickinson, Dr Xiao Wang ORCiD, Dr Shaheda Ahmed

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

© Springer International Publishing AG 2017. A human in vitro skin explant test was developed by Alcyomics for the prediction of adverse immune reactions and assessment of relative potency. The skin explant test has a unique readout of histological damage, which ranges in severity from grade 0 and I (negative reaction) to grades II, III and IV damage (positive reaction) in human full-thickness skin. The test has been assessed for predicting responses to sensitizers and non-sensitizers, originally tested in the mouse local lymph node assay (LLNA). Results compared with the LLNA gave 95% specificity, 95% sensitivity and 95% concordance with a correlation coefficient of 0.9. Similar specificity and sensitivity were achieved for comparison of results to published human data with a correlation coefficient of 0.91. The test can also identify chemicals mis-classified in the mouse local lymph node assay (LLNA). Results of the skin test correlate with high or low T cell proliferation and IFNγ production, and these assays can be used as in vitro screening tests for compounds. Collectively, the human in vitro skin explant test can provide a robust, reliable and novel approach for characterization of sensitizing activity as a first step in the risk assessment process.