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The CarD/CarG regulatory complex is required for the action of several members of the large set of Myxococcus xanthus extracytoplasmic function σ factors

Lookup NU author(s): Dr Javier Abellon-RuizORCiD

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Abstract

Summary: Extracytoplasmic function (ECF) σ factors are critical players in signal transduction networks involved in bacterial response to environmental changes. The Myxococcus xanthus genome reveals ~45 putative ECF-σ factors, but for the overwhelming majority, the specific signals or mechanisms for selective activation and regulation remain unknown. One well-studied ECF-σ, CarQ, binds to its anti-σ, CarR, and is inactive in the dark but drives its own expression from promoter PQRS on illumination. This requires the CarD/CarG complex, the integration host factor (IHF) and a specific CarD-binding site upstream of PQRS. Here, we show that DdvS, a previously uncharacterized ECF-σ, activates its own expression in a CarD/CarG-dependent manner but is inhibited when specifically bound to the N-terminal zinc-binding anti-σ domain of its cognate anti-σ, DdvA. Interestingly, we find that the autoregulatory action of 11 other ECF-σ factors studied here depends totally or partially on CarD/CarG but not IHF. In silico analysis revealed possible CarD-binding sites that may be involved in direct regulation by CarD/CarG of target promoter activity. CarD/CarG-linked ECF-σ regulation likely recurs in other myxobacteria with CarD/CarG orthologous pairs and could underlie, at least in part, the global regulatory effect of the complex on M.xanthus gene expression. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

Publication metadata

Author(s): Abellon-Ruiz J, Bernal-Bernal D, Abellan M, Fontes M, Padmanabhan S, Murillo FJ, Elias-Arnanz M

Publication type: Article

Publication status: Published

Journal: Environmental Microbiology

Year: 2014

Volume: 16

Issue: 8

Pages: 2475-2490

Print publication date: 01/08/2014

Online publication date: 20/02/2014

Acceptance date: 27/12/2013

ISSN (print): 1462-2912

ISSN (electronic): 1462-2920

Publisher: Wiley-Blackwell

URL: https://doi.org/10.1111/1462-2920.12386

DOI: 10.1111/1462-2920.12386

PubMed id: 24428729

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