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Deletion of membrane-associated Asp23 leads to upregulation of cell wall stress genes in Staphylococcus aureus

Lookup NU author(s): Dr Ulrike Mader, Wenke Reiss, Professor Rick Lewis

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Abstract

With about 25000 molecules per cell, Asp23 is one of the most abundant proteins in Staphylococcus aureus. Asp23 has been characterized as a protein that, following an alkaline shock, accumulates in the soluble protein fraction. Transcription of the asp23 gene is exclusively regulated by the alternative sigma factor sigma(B), which controls the response of the bacterium to environmental stress. Sequence analysis identified Asp23 as a member of the widely distributed Pfam DUF322 family, precluding functional predictions based on its sequence. Using fluorescence microscopy we found that Asp23 colocalized with the cell membrane of Staphylococcus aureus. Since Asp23 has no recognizable transmembrane spanning domains, we initiated a search for proteins that link Asp23 to the cell membrane. We identified SAOUHSC_02443 as the Asp23 membrane anchor and have renamed it AmaP (Asp23 membrane anchoring protein). Deletion of the asp23 gene led to an upregulation of the cell wall stress response. In summary, we have identified Asp23 as a membrane-associated protein and we suggest a function for Asp23 in cell envelope homoeostasis.

Publication metadata

Author(s): Muller M, Reiss S, Schluter R, Mader U, Beyer A, Reiss W, Marles-Wright J, Lewis RJ, Pfortner H, Volker U, Riedel K, Hecker M, Engelmann S, Pane-Farre J

Publication type: Article

Publication status: Published

Journal: Molecular Microbiology

Year: 2014

Volume: 93

Issue: 6

Pages: 1259-1268

Print publication date: 01/09/2014

Online publication date: 19/08/2014

Acceptance date: 29/07/2014

Date deposited: 11/07/2016

ISSN (print): 0950-382X

ISSN (electronic): 1365-2958

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1111/mmi.12733

DOI: 10.1111/mmi.12733

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