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Characterization of Microsatellite Loci in Tilia platyphyllos (Malvaceae) and Cross-Amplification in Related Species

Lookup NU author(s): Prattana Phuekvilai, Dr Kirsten Wolff

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).

Abstract

• Premise of the study: Microsatellite markers in the genus Tilia were developed to investigate the genetic variation in T. platyphyllos and its relationship with T. cordata . • Methods and Results: Fifteen microsatellite markers were developed using a microsatellite enrichment protocol. Most loci show a high level of polymorphism in two T. platyphyllos populations from France. The number of alleles ranged from one to 15, with a mean of 8.96. The mean observed and expected heterozygosities were 0.71 and 0.70, respectively. Cross-amplifi cation results indicated that 12 out of 15 loci amplifi ed polymorphic loci in 23 species in the genus. • Conclusions: These markers will be useful tools for investigating the phylogeography and hybridization of Tilia species.

Publication metadata

Author(s): Phuekvilai P, Wolff K

Publication type: Article

Publication status: Published

Journal: Applications in Plant Sciences

Year: 2013

Volume: 1

Issue: 4

Print publication date: 28/03/2013

Date deposited: 25/02/2014

ISSN (electronic): 2168-0450

Publisher: Botanical Society of America

URL: http://dx.doi.org/10.3732/apps.1200386

DOI: 10.3732/apps.1200386

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