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Standardization and quality controls for the methylated DNA immunoprecipitation technique

Lookup NU author(s): Sofia Lisanti, Professor Thomas von Zglinicki, Professor John MathersORCiD

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Abstract

MeDIP (Methylated DNA Immunoprecipitation) is a relatively recent technique aimed to enrich the methylated fraction of DNA with an antibody directed against 5-methyl-cytosine. MeDIP processed samples are suitable for investigation of the methylation status of specific genomic loci and for performing genome-wide screening when hybridized to DNA methylation microarrays or analyzed by deep sequencing. Here, we describe a standardization protocol and quality controls to assess the specificity, reproducibility and efficiency of the MeDIP procedure. These may have utility when comparing results between samples and experiments within laboratories and between laboratories.

Publication metadata

Author(s): Lisanti S, von Zglinicki T, Mathers JC

Publication type: Article

Publication status: Published

Journal: Epigenetics

Year: 2012

Volume: 7

Issue: 6

Pages: 615-625

Print publication date: 01/06/2012

ISSN (print): 1559-2294

ISSN (electronic): 1559-2308

Publisher: Landes Bioscience

URL: http://dx.doi.org/10.4161/epi.20028

DOI: 10.4161/epi.20028

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Funding

Funder referenceFunder name
Biotechnology and Biological Sciences Research Council
Centre for Integrated Systems Biology of Aging and Nutrition
BB/C0082001Engineering and Physical Sciences Research Council
BB/G007993/1Biotechnology and Biological Sciences Research Council
BB/I020748/1Biotechnology and Biological Sciences Research Council

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