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Simple Detection of Protein Soft Structure Changes

Lookup NU author(s): Dr David Allen Chalton, Emeritus Professor Jeremy LakeyORCiD

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Abstract

We present a rapid method for protein tertiary structure analysis which avoids the need for techniques such as circular dichroism and differential scanning calorimetry. Small changes to a protein's noncovalent "soft" structure are detected by exploiting differences in thermal stability and fluorescent reporter binding. It can detect subtle stability differences using micrograms of protein in 2 mu L volumes within minutes.

Publication metadata

Author(s): Chalton DA, Lakey JH

Publication type: Article

Publication status: Published

Journal: Analytical Chemistry

Year: 2010

Volume: 82

Issue: 7

Pages: 3073-3076

Print publication date: 01/04/2010

ISSN (print): 0003-2700

ISSN (electronic): 1520-6882

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/ac902932c

DOI: 10.1021/ac902932c

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Funding

Funder referenceFunder name
BB/F005768/1BBSRC

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